Monday~Sunday
9:00am-17:00pm
No.59, YeHe Roaduff0cYexie Townuff0cSongjiang District Shanghai 201609,P.R.C.
PRODUCTS
Description
Size Exclusion Chromatography (SEC), is a chromatographic method in which molecules in solution are separated by their size, not by molecular weight. It is usually applied to large molecules or macromolecular complexes such as industrial polymers, proteins and nano-particles.
Major brands on the market are TKS, Shodex etc. CNW size exclusion columns have the same excellent performence.
The column used is filled with high-purity silica or polymer containing many pores. Macromolecules which cannot enter the pores flow quickly through the column, while small molecules which can penetrate deep into the pores flow more slowly through the column; other molecules have different retention time arccording to their size.
Compare the calibration curves and select a column that is best suited to the range of molecule weights to be measured. If samples contain molecules larger than the packing material pores, they are excluded and can cause a peak to appear near the exclusion limit.
Size exclusion chromatography can be divided into:
Gel permeation chromatography (GPC) which uses a hydrophobic column packing material and a non-aqueous mobile phase (organic solvent) to measure the molecular weight distribution of synthetic polymers.
Gel filtration chromatography (GFC) which uses a hydrophilic packing material and an aqueous mobile phase to separate, fractionate, or measure the molecular weight distribution of molecules soluble in water, such as polysaccharides and proteins.
Major brands on the market are TKS, Shodex etc. CNW size exclusion columns have the same excellent performence.
The column used is filled with high-purity silica or polymer containing many pores. Macromolecules which cannot enter the pores flow quickly through the column, while small molecules which can penetrate deep into the pores flow more slowly through the column; other molecules have different retention time arccording to their size.
Compare the calibration curves and select a column that is best suited to the range of molecule weights to be measured. If samples contain molecules larger than the packing material pores, they are excluded and can cause a peak to appear near the exclusion limit.
Size exclusion chromatography can be divided into:
Gel permeation chromatography (GPC) which uses a hydrophobic column packing material and a non-aqueous mobile phase (organic solvent) to measure the molecular weight distribution of synthetic polymers.
Gel filtration chromatography (GFC) which uses a hydrophilic packing material and an aqueous mobile phase to separate, fractionate, or measure the molecular weight distribution of molecules soluble in water, such as polysaccharides and proteins.
Review
Review
